Tantárgy adatlapja
The goal of the course is to provide introduction to the structural analysis of biological macromolecules by highlighting the fundamentals and the most recent developments. Topics include:
Fundamentals of protein structure
Fundamentals of the 3D structure of nucleic acids
Low-resolution structure analysis methods: circular dichroism, FT-IR, UV-VIS, SAXS
Sample preparation for structural studies
Macromolecular X-ray crystallography
Macromolecular NMR spectroscopy
Solid-state NMR for the investigation of biomolecules
Cryo-electron microscopy
Fluorescence-based methods to probe the structure and dynamics of proteins and complexes
Single-molecule methods
Fundamentals of molecular modeling
Basics of molecular dynamics calculations
Hybrid methods combining calculations with experimental data
Interpretation of protein structures to understand biological function
List of selected publications:
P. Moore: Visualizing the Invisible. Oxford University Press, 2012, ISBN: SBN13 9780199767090 I. Bertini, KS McGreevy, G. Parigi: NMR of biomolecules. Wiley-Blackwell, 2012, ISBN:9783527328505. DC Rapaport: The art of molecular dynamics simulation. Cambridge University Press, 2004, ISBN: 978-0521825689 ID Campbell: Biophysical techniques. Oxford University press, 2012. ISBN 978019964214 List of required professional competences: Understand and interpret biomacromolecular structures, design experiments to probe functionally important features of proteins
